Rongshou Chen 1, Mina Mozaffarian 1,2, Negar Habibyar 3,*
1 School of Life Sciences, Xiamen University, Xiamen, China
2 Faculty of Science, Urmia Payam Nour University, Urmia, Iran
3 Faculty of Chemical Engineering, Mazandaran University, Mazandaran, Iran
Corresponding author:
Negar Habibyar, Faculty of Chemical Engineering, Mazandaran University, Mazandaran, Iran.
Email addresses: negar.habibyar@gmail.com
Abstract
One of the fast-growing areas in recent years is the bone tissue engineering field that offers novel and brilliant techniques for bone regeneration. The purpose of the current work is to test the cell growth behavior of starch/collagen electrospun nanofibers on human osteoblast-like osteosarcoma SaOs-2 cell line. The morphology images of cell culture results showed that cells covered nanofibers after 21 days of culture. It seems that the cell population growing on the scaffolds offered an ability to bridge between fibers.
Keywords: Bone tissue engineering, Nanofiber, Osteosarcoma.
Introduction
Natural origin and high biocompatibility alter the starch-based materials to the helpful materials in the biomedical zone [1]. Collagen also as the main constituent of connective tissue has shown outstanding biocompatibility when used in tissue engineering [2]. On the other hand, bone tissue engineering as a fast developing investigation zone proposes new and hopeful methods for bone repair and regeneration. Different necessities have been described for engineering bone, such as selecting a cell type that matures/ differentiates into bone cells with the appropriate form and phenotype, regulating the growth factors and developing a perfect scaffold [3].
Human osteoblast-like osteosarcoma SaOs-2 cell line is frequently used in bone cancer study as a model for presenting of new treatments. In addition, their universal accessibility causes well-documented characterization data about this cell line. The option to gain large amounts of cells in a short time, and fully differentiation in a manner that the osteoblastic cells naturally do are other advantages of this cell line. The ability of Saos-2 cells in mineralization of the extracellular matrix converts these cells to a valuable model for testing of osteoblastic differentiation stage in human cells [4,5].
Electrospinning is an electrostatic-based one-step technique to yield fibers or particles using different types of materials. Lately, fibrous scaffolds in the arena of tissue engineering have been one of the main groups of synthetic biodegradable polymer-based materials. Many applications of electrospun fibers have been appeared to be the consequence of their unique properties, mainly large surface-area-to-volume ratio [6,7].
Materials and methods
All the reagents used were analytical grade and were prepared from Sigma-Aldrich, Germany. The human osteosarcoma cell line (SaOS-2) was prepared from National Cell Bank, Pasteur Institute, Tehran, Iran.
Cell culture experiment
The starch/collagen electrospun nanofiber scaffolds (were prepared in our previously unpublished work) were tested on human osteoblast-like osteosarcoma SaOs-2 cell line. Briefly, the cells were seeded onto the structures using a density of 3×105 cells/scaffolds and permitted to grow for 3 weeks, with medium (DMEM low glucose supplemented with 10% Foetal Bovine Serum, 1% antibiotics/ antimycotics changes every 2 days.
Results and discussion
The electrospinning process was used to optimize the situations of manufacturing fibers with minimum diameter and proper physicochemical properties. Optimum settings in our work were: 60/40 wt% of starch/ collagen, 15 kV voltage, the distance of 10 cm between a collector, a capillary tube for 15 s and 0.8 ml/h flow rate. The characterization of scaffolds in our previous work showed nanosized diameter (120.34 nm) and a network structure without the presence of particles or beads with the surface charge of -15 ± 0.19 mV, representative of their stability. The cell culture results showed that cells covered nanofibers after 21 days of culture. It seems that the cell population growing on the scaffolds offered an ability to bridge between fibers [8]. The authors believed that the processing parameters were effectively optimized for producing fine nanofibers.
Conclusion
The purpose of the current work is to test the cell growth behavior of starch/collagen electrospun nanofibers on human osteoblast-like osteosarcoma SaOs-2 cell line. The main goal was to introduce cheap and available polymer-based biomaterials for bone tissue engineering purposes. The cell culture results showed that cells covered nanofibers after 21 days of culture. Then, the nanofibers can be used as tissue engineering materials.
Conflict of interest
The authors declare that they have no conflict of interest.
Acknowledgment
Urmia Payam Nour University as financial support is acknowledged.
References
HOW TO CITE
Chen, R., Mozaffarian, M., & Habibyar, N. (2019). The cell growth behavior of starch-based electrospun nanofibers on human osteoblast-like osteosarcoma SaOs-2 cell line. Journal of Advanced Chemical and Pharmaceutical Materials (JACPM), 2(1), 109-110. Retrieved from http://advchempharm.ir/journal/index.php/JACPM/article/view/82
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